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. 2014 Aug;95(Pt 8):1755–1769. doi: 10.1099/vir.0.064303-0

Fig. 7.

Fig. 7.

ORF65 polypeptides that contain mutations of the A54 residue do not bind to the MCP efficiently. Sf21 cells were infected as described in the legend to Fig. 1(d). The lysates were immunoprecipitated with anti-GFP antibodies, and the co-precipitated (top panel) and input proteins (two bottom panels) were revealed by immunoblot using anti-V5 (αV5) and anti-GFP (αGFP) antibodies. Lane 1, WT ORF65CGFP; lane 2, ORF65-A54KCGFP; lane 3, ORF65-Δ50–55CGFP. Molecular mass standards (kDa) are indicated on the left.