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. 2014 Aug;95(Pt 8):1755–1769. doi: 10.1099/vir.0.064303-0

Table 1. Primer list.

Primer Sequence
ORF65-SpeI-F GGACTAGTACCATGTCCAACTTTAAGGTGAGAGAC
ORF65-Hind3amb-R GGAAGCTTTTTCTTTTTGCCAGAGGGGGG
ORF65-CHA-Hind3-R GGAAGCTTCTATGCGTAGTCCGGAACGTCGTATGGGTATTTCTTTTTGCCAGAGGGGGGTTTCCTCGC
ORF65-CV5-Hind3-R GGAAGCTTCTACGTAGAATCGAGACCGAGGAGAGGGTTAGGGATAGGCTTACCTTTCTTTTTGCCAGAGGGGGGTTTCCTCGC
EGFP-Hind3-F GGAAGCTTATGGTGAGCAAGGGCGAGGAGCTG
EGFP-Hind3-R GGAAGCTTCTACAGCTCGTCCATGCCGAGAGTGATC
ORF65ΔEcoRI-F CTGAGGAGGATGGGTGGGATTCAAAGGCGGGAT
OR65-EcoRI-F GGAATTCACCATGTCCAACTTTAAGGTGAGAGAC
ORF65-SpeIamb-R GGACTAGTTTTCTTTTTGCCAGAGGGGGG
ORF65C6XHIS-SpeI-R CACTAGTCTAGTGGTGGTGGTGGTGGTGACTGCCTTTCTTTTTGCCAGAGGGGGGTTTC
pFastBac Dual primers
ORF26-XhoI-F GCTCGAGACCATGGCACTCGACAAGAGTATAGTG
ORF26-KpnI-R GGGGTACCTTAGCGTGGGGAATACCAACAGGAG
ORF17.5-XhoI-F GCTCGAGACCATGAACAGCTCTGGTCAAGAGG
ORF17.5-KpnI-R GGGGTACCCTACTGCTTGTTCAGGAGCTCCTC
ORF65-XhoI-F GGCTCGAGACCATGTCCAACTTTAAGGTGAGAGAC
ORF65-KpnI-R GGGGTACCCTATTTCTTTTTGCCAGAGGGGGG
ORF65C6XHIS-KpnI-R GGGGTACCCTAATGGTGATGGTGATGGTGTCCTCCGCCTTTCTTTTTGCCAGAGGGGGGTTTCC