Table 1.
PCR parameters for amplification of NPY, AgRP, POMC, CART mRNAs under basal conditions.
| Transcript | Sequence (3′-5′) forward (f) ad reverse (r) | Product size (bp) | [Mg2+] (mM) | Annealing temperature | Number of cycles | Genbank accession number |
|---|---|---|---|---|---|---|
| NPY | f: gcc atg atg cta ggt aac aaa r: tct ctt gcc ata tct ctg tct |
214 | 1.5 | 55°C | 30 | M20373 |
| AgRP | f: gct gca gaa ggc aga agc r: tga aga agc ggv agt agc ac |
152 | 1.5 | 58°C | 35 | AF206017 |
| POMC | f: cgg ccc cag aaa cag cag cag t r: ggg ccc gtc ggc ctt ctc g |
304 | 1.5 | 66°C | 30 | NM_139226 |
| CART | f: gat cgg gaa gct gtg tga ct r: att ttg aag cag cag gga aa |
208 | 1.5 | 56°C | 31 | NM_017110 |