Table 1.

Characteristics of 12 polymorphic cpSSR markers developed in Vigna unguiculata.

Locus	Repeat motif	Primer sequences (5′–3′)	Ta (°C)	Positiona	Region	GenBank accession no.	Size range in V. unguiculata (bp)	Size range in P. vulgaris (bp)
VgcpSSR1	(TA)5	F: GGTGGATGTTTATACCCAATCG	60	trnK-rbcL IGS	LSC	KF662476	190–220	190–196
		R: TCTTTCTGCGATACAAACAAGAA
VgcpSSR2	(AAT)5	F: TTTTCTATGTATGGCGCAACC	60	rbcL-atpB IGS	LSC	KF662477	180–190	186–190
		R: CGGGGATAAAGCTGCCTATT
VgcpSSR3	(TA)12	F: AAACCACTCGAATATTATGGAAA	57	ndhJ-trnF IGS	LSC	KF662478	185–305	265–355
		R: CCAGTTCAAATCTGGTTCCTG
VgcpSSR4	(AT)5	F: GAAAAGAACAAGCAAATCCACA	60	ycf3 exon	LSC	KF662479	180–280	180–280
		R: TGATCCTTACGATGCTTCCTTT
VgcpSSR5	(TA)5	F: AGCCCACTTTTCCGTAGGTT	58	psaB-rps14 IGS	LSC	KF662480	190–202	190–202
		R: CTTTTCCTTGCCATAATGGTT
VgcpSSR7	(TA)6	F: TCAACCATTTCCCAACACCT	59	psbD-trnT IGS	LSC	KF662481	136–196	196
		R: CATCGAGTTCATGGATTTGC
VgcpSSR9	(TA)5	F: TGAAATTTGAAAAACGGGGTA	57	trnR-trnS IGS	LSC	KF662482	144–156	160
		R: AAGCGATACGGATAGATTCCT
VgcpSSR10	(AT)5	F: GGGCTCATTGGCTGTAGAAA	59	trnR-trnS IGS	LSC	KF662483	150–182	182–186
		R: CCATCTCTCCCCAATTGAAA
VgcpSSR11	(AT)6	F: TTTGAGAAGGTTCAATTGTTCG	59	petA-psbJ IGS	LSC	KF662484	168–186	168–170
		R: TCGGACTCTAGGAAAGGACAA
VgcpSSR12	(AT)6	F: GGCCATTTATCCCACTTTCC	56	psbJ-psbL-psbF IGS	LSC	KF662485	162–220	170–220
		R: CCAGTCTCTACTGGGGGTTA
VgcpSSR13	(TA)5	F: TATTGGTTTTGCACCAATCG	60	rpl20-rps12 IGS	LSC	KF662486	162–210	210
		R: ACCAGGGTGTATGTGCGACT
VgcpSSR14	(AT)5	F: TGGATCATAATCCTTGAACATCA	59	psaC-ndhE IGS	SSC	KF662487	162–210	178–180
		R: TGCGAAAACAAAGATAAGAAATCA

Note: IGS = intergenic spacer; LSC = long single-copy region; SSC = short single-copy region; T_a = annealing temperature.

^aPosition of each SSR in chloroplast complete genome of Vigna unguiculata (GenBank accession number: NC_018051).