Table 1.
Characteristics of 14 novel genomic microsatellite markers developed in Gleditsia triacanthos.a
| Locus | Primer sequences (5′–3′)b | Amplicon size (bp) | Repeat motif | Ta (°C) | Size range (bp) |
| GLT002 | F: NED-TAAAAAGTAACCTTAAAGG | 104 | (AT)9 | 56 | 103–147 |
| R: AGTAAAGAGGTAACGATTT | |||||
| GLT021 | F: 6-FAM-ATATCACCAATTTAAGACC | 100 | (AG)11 | 56 | 94–98 |
| R: GTACACAAAACTTCGAGAG | |||||
| GLT026 | F: VIC-AAGCTTGATTAGAGAAATT | 127 | (AT)14 | 56 | 113–143 |
| R: AGATAGTTCCTTTCAGTTG | |||||
| GTT057 | F: PET-CAGGTAAAACATGAGATTGATGC | 121 | (TA)9 | 56 | 127–157 |
| R: TTCCATAAAATCAGTCATGCAA | |||||
| GTT063 | F: NED-CTCTTGCGCACACTAAAACG | 116 | (AC)12 | 56 | 147–187 |
| R: CGTACGGTGACACTTGTGC | |||||
| GTT073 | F: VIC-CATGATTTAGAGAGAGAAATGTTTTGG | 109 | (GA)13 | 56 | 134–164 |
| R: AACCAAGCCCTTCATTTATGG | |||||
| GTT114 | F: 6-FAM-TCAAGCTAGTTAGCCTTCCTGC | 121 | (TC)20 | 56 | 102–138 |
| R: AAATATGGGAGCAATGAACC | |||||
| GTT116 | F: NED-CTAAAGCTTGACTTCTGAATCC | 134 | (CT)8 | 56 | 131–143 |
| R: CGCTATATCGGAATCCCTGC | |||||
| GTT117 | F: PET-GGTGGTATGTGCAAGCAAGC | 120 | (TA)8 | 56 | 110–122 |
| R: CTTGAGCCACCCATTACCC | |||||
| GTT118 | F: 6-FAM-CAGTCCCACCTTCACTAGCC | 119 | (CT)8 | 56 | 110–130 |
| R: TGCGTGTAATCTGAGCTTGG | |||||
| GTT126 | F: PET-TGGATTAAGTTGTAAAGCGAGG | 109 | (AT)8 | 56 | 98–146 |
| R: CCGTCAAACTTAAGACCCACC | |||||
| GTT131 | F: 6-FAM-CTTTGAACTCTAATACTCTGGTTGC | 100 | (AC)9 | 56 | 91–153 |
| R: TCAACCACCTTAAGACATCCC | |||||
| GTT132 | F: VIC-CAGTCCTCATGTCTAGTCTAGTGC | 105 | (AT)11 | 56 | 90–130 |
| R: CAATCTCTGGTGCAAGATGC | |||||
| GLT4027 | F: 6-FAM-AGGAATTATTCTCTACCAA | 107 | (TCCA)6 | 56 | 91–107 |
| R: CGAATCTCATTTTATACAA |
Note: Ta = annealing temperature.
a
Geographic coordinates for the provenances are given in Appendix 1.
b
The fluorescent label is shown with the forward primer.