Appendix 1.

Geographic coordinates of Gleditsia triacanthos seed parent (784) and potential pollen parents (Butternut Valley, Memphis, Tennessee, USA) used for microsatellite marker screening.

Accession no.	Locality	Latitude	Longitude
784*	DeKalb, Tennessee, USA	35°54′46.607″N	85°54′32.083″W
780	DeKalb, Tennessee, USA	35°54′47.090″N	85°54′31.678″W
781	DeKalb, Tennessee, USA	35°54′47.090″N	85°54′31.678″W
782	DeKalb, Tennessee, USA	35°54′47.256″N	85°54′32.114″W
783	DeKalb, Tennessee, USA	35°54′46.697″N	85°54′31.244″W
785	DeKalb, Tennessee, USA	35°54′51.779″N	85°54′29.778″W
786	DeKalb, Tennessee, USA	35°54′50.392″N	85°54′30.755″W

^*Individual used to make the Illumina library.