Table 1.
Characteristics of 11 microsatellite loci of Elaeagnus angustifolia.
| Locus | Primer sequences (5′–3′)a | Repeat motif | Size range (bp) | Ta (°C) | GenBank accession no. |
| Ro20 | F: TCCCATACCCAGCACAAGG | (AAG)6 | 233–272 | 56 | KC516766 |
| R: CAGTCGGGCGTCATCAGCCATGAGGAATTGAAGGGTG | |||||
| Ro97 | F: GGAAACAGCTATGACCATCGGACTGCTTTGAACCCTC | (ACC)6 | 160–199 | 53 | KC516767 |
| R: AAGCCACTCAGCCTATCTAC | |||||
| Ro218 | F: CAGTCGGGCGTCATCAGTGGAAATGCGCTGGATCG | (AAAG)12 | 358–390 | 56 | KC516768 |
| R: AACCCAAGTCCAAATGCCC | |||||
| Ro236 | F: GTGCCTTATTCCTCGCCAC | (AGCCTC)4 | 269–299 | 56 | KC516769 |
| R: GGAAACAGCTATGACCATCCAAATGCAGGCCCAAAGG | |||||
| Ro325 | F: GGAAACAGCTATGACCATCCAAGAGCAGCGCATTC | (ACC)4 | 198–210 | 54 | KC516770 |
| R: ACTGGCCGGTGACAAGG | |||||
| Ro367 | F: CAGTCGGGCGTCATCAGTAGGAAGTTGGAGCTCTGC | (AG)13 | 330–360 | 55 | KC516771 |
| R: GTTGGAATTGCCGGTTGGG | |||||
| Ro721 | F: CAGTCGGGCGTCATCAGGGTGGTCCTCTGACATTTG | (ACC)7 | 271–286 | 55 | KC516772 |
| R: TGCGCTCTGTATGGAATGC | |||||
| Ro749 | F: CAGTCGGGCGTCATCAGGTACCACGGTTCAGTGTATTTC | (AAGAAT)5 | 195–225 | 55 | KC516773 |
| R: CACCAAACGGGCATTCTCC | |||||
| Ro833 | F: GGGAGCTTACCAACCTTGC | (AAG)7 | 264–300 | 54 | KC516774 |
| R: GGAAACAGCTATGACCATGCAATCGAAGTCCGTGC | |||||
| Ro851 | F: GGTCTAAGAGCTTCAAGTGGC | (AAAAT)4 | 244–254 | 52 | KC516775 |
| R: CAGTCGGGCGTCATCAGGAGATTCTGACCTGTGATAAG | |||||
| Ro887 | F: CAATCGCGAGTAGGGAACC | (AAG)5 | 355–373 | 53 | KC516776 |
| R: CAGTCGGGCGTCATCACCCATTGATTAACTGTACACCTTG |
Note: Ta = annealing temperature.
a
The forward and reverse primer sequences are shown with added tails set in boldface.