Induction of IL-10 protein and mRNA in BMDMs. A, Total of 0.5 × 106 WT, IFN-αR KO, and IL-27R KO BMDMs were stimulated with LPS (50 ng/ml), and supernatant was collected at the indicated time points. IL-10 protein production was quantified via ELISA. B, Total of 0.5 × 106 BMDMs were unstimulated or stimulated with LPS (50 ng/ml), IFN-α (250 U/ml), or IL-27 (10 ng/ml), supernatant was collected, and IL-10 protein production was quantified via ELISA. C, Total of 0.5 × 106 BMDMs were unstimulated or stimulated with LPS (50 ng/ml), IFN-α (250U/ml), or IL-27 (10 ng/ml) for 4 h, RNA was harvested, and relative IL-10 transcript was detected via qPCR normalized to L32.