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. Author manuscript; available in PMC: 2014 Jul 18.
Published in final edited form as: J Immunol. 2010 Nov 1;185(11):6599–6607. doi: 10.4049/jimmunol.1002041

FIGURE 1.

FIGURE 1

Induction of IL-10 protein and mRNA in BMDMs. A, Total of 0.5 × 106 WT, IFN-αR KO, and IL-27R KO BMDMs were stimulated with LPS (50 ng/ml), and supernatant was collected at the indicated time points. IL-10 protein production was quantified via ELISA. B, Total of 0.5 × 106 BMDMs were unstimulated or stimulated with LPS (50 ng/ml), IFN-α (250 U/ml), or IL-27 (10 ng/ml), supernatant was collected, and IL-10 protein production was quantified via ELISA. C, Total of 0.5 × 106 BMDMs were unstimulated or stimulated with LPS (50 ng/ml), IFN-α (250U/ml), or IL-27 (10 ng/ml) for 4 h, RNA was harvested, and relative IL-10 transcript was detected via qPCR normalized to L32.