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. Author manuscript; available in PMC: 2015 Jun 5.
Published in final edited form as: Mol Cell. 2014 May 22;54(5):777–790. doi: 10.1016/j.molcel.2014.04.025

Figure 5. LincRNA-p21 regulates p21in cis.

Figure 5

(A) Single molecule RNA FISH detection of lincRNA-p21 with exon-specific probes in indicated MEFs (white arrows). DNA was counterstained with DAPI. % represents the fraction of cells with indicated number of signals per cell (n=150).

(B) Co-localization of exon- and intron-specific lincRNA-p21 signals using single molecule RNA FISH.

(C) (Top) Schematic. (Bottom) qRT-PCR analysis of lincRNA-p21 and p21 RNA levels in MEFs of indicated genotypes and treatments. Data are represented as mean±SEM, n=3, p<0.0001, paired t-test.

(D) qRT-PCR analysis of lincRNA-p21 (top) and p21 (bottom) RNA levels in MEFs of indicated genotypes, expressing TRE-lincRNA-p21, in the presence or absence of doxorubicin (Doxo) and doxycycline (Doxy). Data, replicated in 2 independent experiments, are represented as mean±SEM of technical replicates.

(E) Single molecule RNA-FISH detection of exogenously expressed lincRNA-p21 in (D).See also Table S4.