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. Author manuscript; available in PMC: 2015 Jan 1.

Published in final edited form as: J Cardiovasc Pharmacol Ther. 2013 Nov 28;19(1):121–132. doi: 10.1177/1074248413508003

Lack of ROS-scavenging capacity of Bendavia in in vitro, cell-free systems. The ability of Bendavia to scavenge ROS (specifically H₂O₂) was tested with either a static H₂O₂ incubation (A), or a dynamic glucose-glucose oxidase reaction, which generates H₂O₂ as a reaction product (B). The net amount of H₂O₂ produced under each condition in B was calculated after 30 minutes of the glucose-glucose oxidase protocol, and are presented in (C). H₂O₂ levels were assessed by Amplex Red fluorescence. Bendavia did not influence H₂O₂ levels across a wide range of concentrations tested, in clear contrast to the positive control catalase. D. Additionally, the superoxide (O₂^•−) scavenging capacity of Bendaiva was tested using a xanthine-xanthine oxidase reaction. Bendavia also showed no observable scavenging capacity for O₂^•−, in contrast to the positive control superoxide dismutase (SOD). *, P<0.05 (ANOVA).

Lack of ROS-scavenging capacity of Bendavia in in vitro, cell-free systems. The ability of Bendavia to scavenge ROS (specifically H₂O₂) was tested with either a static H₂O₂ incubation (A), or a dynamic glucose-glucose oxidase reaction, which generates H₂O₂ as a reaction product (B). The net amount of H₂O₂ produced under each condition in B was calculated after 30 minutes of the glucose-glucose oxidase protocol, and are presented in (C). H₂O₂ levels were assessed by Amplex Red fluorescence. Bendavia did not influence H₂O₂ levels across a wide range of concentrations tested, in clear contrast to the positive control catalase. D. Additionally, the superoxide (O₂^•−) scavenging capacity of Bendaiva was tested using a xanthine-xanthine oxidase reaction. Bendavia also showed no observable scavenging capacity for O₂^•−, in contrast to the positive control superoxide dismutase (SOD). *, P<0.05 (ANOVA).