FIG. 2.

Blockage of MSC-EV incorporation by PTECs. MSC-EVs stained with Vybrant Dil (red) were incubated for 24 h with PTECs submitted to ATP depletion injury. To block CD29 and CD44 integrins the MSC-EVs were previously incubated with anti-CD29 antibody, hialuronic acid (HA), or both simultaneously as indicated in the panels. Left panels indicate internalization of MSC-EV membrane. INJ indicated cells submitted to injury without any blockage. Middle panels show the nuclei of PTECs stained with DAPI (blue). Right panels show a merge of the two previous images. Three experiments were performed with similar results using MSC-EVs derived from different MSCs. Original magnification: ×630.