FIG. 5.

Changes in the expression of predicted miRNA targets modulated by MSC-EVs. Evaluation of the gene expression of miRNA targets predicted by GO analysis related to hypoxia, cytoskeleton reorganization, and apoptosis processes. The changes in gene expression were performed by quantitative real-time polymerase chain reaction. The analysis was performed in all three conditions: control (white bars), injury (black bars), and injury treated with MSC-EVs (gray bars). Data are expressed as RQ, normalized to GAPDH. The abscissa indicated the evaluated genes. Statistical analysis was performed by ANOVA with Dunnett multicomparison test: *statistical difference to the control group; ^#statistical difference to the injured group (P<0.05; n=4).