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. 2014 Mar 20;23(15):1765–1776. doi: 10.1089/scd.2013.0628

FIG. 4.

FIG. 4.

Positional and retinal identity of induced cells. (a–b) qRT-PCR results (n=3) at the end of the 2−/4+ induction showing mRNA levels for positional Hox genes compared with control cultures induced with 1 μM Pur and 0 nM RA. (c) qRT-PCR results (n=3) at the end of the 2−/4+ induction showing mRNA levels for the photoreceptor progenitor transcription factor Crx compared with control cultures induced with 1 μM Pur and 0 nM RA. Dotted line denotes downregulation. The symbol & denotes significance over 10 nM, 50 nM, 100 nM, and 2 μM groups (P<0.05). The symbol ^ denotes significance over 10, 50, and 100 nM (P<0.05). The symbol * denotes significance over 10 and 2 μM groups (P<0.05). The symbol # denotes significance over 10 μM group (P<0.05). Error bars denote SEM. Analysis was performed using Scheffe's post hoc test (n=3).