FIG. 6.

Staining of dissociated cultures. Cultures induced with the 2⁻/4⁺ protocol with 1 μM Pur, 10 nM RA, and 5 μM DAPT. Cultures were dissociated and stained with antibodies for Chx10, Lhx3, Hb9, and β-tubulin III (B-Tub), a neuronal marker. (a–c) Chx10 costained with B-Tub and DAPI. (f–h) Lhx3 costained with B-Tub and DAPI. (k–m) Hb9 costained with B-Tub and DAPI. The above images were overlayed (d, i, n) and enlarged to show neurite extension (e, j, o). Scale bars are 100 μm. Color images available online at www.liebertpub.com/scd