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. 2014 Jun 23;111(27):9941–9946. doi: 10.1073/pnas.1311685111

Fig. 5.

Fig. 5.

Effects of rhIGF1 on Mecp2−/+ breathing patterns, anxiety, and spatial recognition. (A) Representative breathing pattern traces of WT (Top) and Mecp2−/+ females before (Middle) and after (Bottom) treatment. Downward indicates inspiration (Fig. S4C). (B) Average inspiratory (Ti) and expiratory (Te) times were abnormal in Mecp2−/+ females, and treatment restored these to WT levels (paired t test for post/pre comparison and ordinary one-way ANOVA with Tukey’s post hoc analysis for multiple comparisons). Dots represent individual animals. (C) Total number of breath holds observed during 12-min recording sessions pre- and posttreatment (paired t test). Inset shows representative breath hold trace from a pretreatment female. (Scale bar, 500 ms.) (D) Performance ratio of time spent with displaced versus nondisplaced objects (see Materials and Methods for details of assay) pre- and posttreatment (paired t test). (E) Percent time spent in the open arms of elevated plus maze pre- and posttreatment (paired t test). (F) Relative serum levels of total IGF1 (murine and rhIGF1) measured pre- and posttreatment (one-way ANOVA with Tukey’s post hoc analysis for multiple comparison). Error bars represent S.E.M. *P < 0.05; **P < 0.01; ****P < 0.0001. In C–E, dashed line and gray shading represent mean and SEM of WT mice, respectively. See also Fig. S4.