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. 2014 Jun 23;111(27):9947–9952. doi: 10.1073/pnas.1309426111

Fig. 1.

Fig. 1.

Treatment with clenbuterol substantially increases survival and improves behavior of Mecp2 KO mice. (A) Schematic showing the duration of clenbuterol treatment and time of behavioral assays in Mecp2−/y mice. (B) Graph showing cumulative survival distributions for KO vehicle [KO (v), red circles], and KO clenbuterol-treated [KO (t), green circles] mice. P value shown in graph is based on Gehan–Breslow–Wilcoxon test. (C) Graph showing mean ± SEM latency to fall rotarod motor coordination test values (in seconds) in WT vehicle [WT (v), black circles], KO vehicle [KO (v), red circles], and clenbuterol-treated [KO (t), green circles] mice during the second experimental day. (D) Graph showing mean ± SEM breath rate values (breaths per minute) for the three experimental groups. Stars in C and D depict statistical significance based on ANOVA with Newman–Keuls test for multiple comparisons (***P < 0.001, **P < 0.01, *P < 0.05). (E) Graph showing mean ± SEM percentage of time spent in the chamber with the Stimulus mouse for WT (v) (n = 29), KO (v) (n = 9), and KO (v) (n = 11) during the first (social approach or SA, filled bars) and second day (social recognition or SR, bars with diagonal lines) of the three-chamber test. The asterisks denote statistical significance based on two-tailed paired t test (***P < 0.001, **P < 0.01, with the exact P values depicted in the figure).