Fig. 4.
Degradation of SNA-1. (A) Plot of the ratio of AS oligonucleotide per AuNP for SNA-1 over time. The τ1/2 of the AS oligonucleotide is ∼2 min in 10% FBS at 37 °C (fit to a first order exponential decay function), whereas in PBS and deactivated FBS at 37 °C, the ratio is indistinguishable from that of the starting material. When SNA-1 is incubated in 10% FBS at 20 °C or 10% FBS with ribonucleoside vanadyl complex (an RNase A inhibitor), τ1/2 of the AS oligonucleotide is 15 min and 30 min, respectively. Error bars represent the range for n = 4 measurements. (B) Denaturing PAGE of the AS oligonucleotide from SNA-1 after 10 min in 10% FBS and 1× PBS (lanes i and ii, respectively) and analytical standards (lane iii).