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Binding of Lb-Tec2 to C. elegans N-glycans. (A) Silver-stained SDS/PAGE gel and lectin blot of protein extracts from C. elegans pmk-1(km25) (wild type) and samt-1(op532)pmk-1(km25) (samt-1). (B) Silver-stained SDS/PAGE gel and lectin blot of protein extracts from C. elegans fut-6(ok475)fut-1(ok892) after PNGase F digest. Dashes indicate negative control not incubated with the lectin. Asterisks indicate unspecific bands. m, marker.
