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. 2014 May 30;111(27):E2787–E2796. doi: 10.1073/pnas.1401176111

Fig. 4.

Fig. 4.

MALDI-TOF spectra of deuteromethylated wild-type and mutant C. elegans N-glycans released by PNGase A digestion. N-glycans were released from C. elegans tryptic glycopeptides by digestion with PNGase A after an initial PNGase F digest (see also SI Appendix, Fig. S5). Released glycans were deuteromethylated before analysis; all molecular ions are [M+Na]+. Structural assignments are based on monosaccharide composition, MS/MS fragmentation analyses, and knowledge of the glycan biosynthetic pathways. (A) N-glycans released from C. elegans pmk-1(km25) (WT), m/z 1,700–2,500. (B) N-glycans released from C. elegans pmk-1(km25), m/z 1,990–2,020. (C) N-glycans released from C. elegans samt-1(op532)pmk-1(km25) (samt-1), m/z 1,700–2,500. (D) N-glycans released from C. elegans samt-1(op532)pmk-1(km25), m/z 1,990–2,020.