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. 2014 May 30;111(27):E2787–E2796. doi: 10.1073/pnas.1401176111

Fig. 7.

Fig. 7.

Conservation of O-methylation–dependent bacterial agglutination and nematotoxicity between Lb-Tec2 and limulus lectin L6. (A) Soluble extract of E. coli ArcticExpress expressing either Lb-Tec2 (as positive control) or L6 was added to E. coli O9a:K−, 08:K−, or O8−:K− in a 96-well plate. The soluble extract of E. coli ArcticExpress harboring the empty vector plasmid and Hepes buffer was used as negative controls. (B) Larval development of C. elegans pmk-1(km25) (wild type) and samt-1(op532)pmk-1(km25) (samt-1) was assayed as previously described but using E. coli ArcticExpress containing the empty vector (pET24a) or expressing Lb-Tec2 (pET24-Lb-Tec2) or limulus lectin L6 (pET24-L6) as food (n = 5). Error bars indicate SD. Comparisons between the two C. elegans strains for each plasmid-containing bacterium were performed using the Mann–Whitney U test (**P < 0.01).