Mitochondrial protein expression profile in SD (Sprague–Dawley) and SHHF lean and obese rats with and without exercise training. Aortic lysates were generated from SD (n = 8 and 9 for sedentary and exercise respectively), SHHF lean (n = 8 and 10 for sedentary and exercise respectively) and SHHF obese (n = 9 for both sedentary and exercise) rats as labelled: 30 µg of protein was run on SDS/PAGE (10% gels), transferred on to nitrocellulose membranes and Western blot analysis was carried out. Results are means±S.E.M. *P < 0.05; †P=0.05–0.10 (Student’s t test). Reproduced with permission from [36]: Knaub, L.A., McCune, S., Chicco, A.J., Miller, M., Moore, R.L., Birdsey, N., Lloyd, M.I., Villarreal, J., Keller, A.C., Watson, P.A. and Reusch, J.E. (2013) Impaired response to exercise intervention in the vasculature in metabolic syndrome. Diab. Vasc. Dis. Res. 10, 222–238. © 2013 SAGE Publications.