Table 1.

Impact of small molecule injections on maize plants at the 2–5-cm tassel stage

Treatment	Injection site	Upper leaves	Tassel	Notes
Water	Holes heal	No effect	No effect
Mock infection	Tiny chlorosis	Chlorosis	No effect	See Fig. 1a
1 μM Auxin	Tiny chlorotic halo	No effect	No effect
1 μM CPD	Tiny chlorotic halo	No effect	No effect
1 μM Gibberellic acid	Tiny chlorotic halo	No effect	No effect
1 μM Cytokinin	Tiny chlorotic halo	No effect	No effect
1 μM Abscisic acid	Tiny chlorotic halo	No effect	No effect
1 μM Methyl jasmonate	Extensive chlorosis and necrosis	Chlorosis and necrosis	Pale tissue zone with arrested anthers	See Fig. 5b
1 nM Brassinosteroid	Extensive chlorosis	Extensive chlorosis	Browning zone with arrested anthers	See Fig. 5c

Greenhouse and field-grown W23 plants were used for each treatment (N = 8–36 per compound) with three trials of MeJ and BR on W23 and one trial on A619 (N = 50 plants each). CPD is a non-hydrolyzable 1-naphthylphthalamic acid analog that inhibits polar auxin transport (Geisler et al. 2005)