Skip to main content
. 2014 Jun 4;2(6):apps.1400024. doi: 10.3732/apps.1400024

Table 1.

Description of 11 microsatellite loci for Parkia biglobosa developed from 10 samples from Burkina Faso.a

Locus Primer sequences (5′–3′) Repeat motif Allele size range (bp)b Fluorescent labelc GenBank accession no.
PbL02 F: CGAAATAAGAACTCGGACCAAA (GA)17 180–205 NED KJ475533
R: ATGCCGTGTTCTGTTTCACC
PbL03 F: TTCGATTCAATTCAACTTGCAG (GA)17 90–138 6-FAM KJ475534
R: TCGGTGCTAGCAATATCAGC
PbL04 F: GAAGCCTTGGAATGAAGTTGA (CA)17 161–181 VIC KJ475535
R: GAAAACGGAAGGCATGGTTA
PbL05 F: GAATCAGAGAAGCCCTTAGGTT (AC)17 183–259 PET KJ475536
R: GCCGCTTGTTTTCTTGTGA
PbL09 F: TGACGTATTTGAGTGTCTTTTACACA (AG)18 126–170 6-FAM KJ475537
R: GCAGAAAATCACAAATGCAGA
PbL11 F: TCACGGCAATAGAACTCATCA (CCT)18 170–206 PET KJ475538
R: ACGGAGCAGGATGAAGTCAG
PbL12 F: ATCTAGGCATCCATGCAAATG (TG)19 108–137 VIC KJ475539
R: CAGAAGTTGTTAGACAATTATGGTGA
PbL15 F: CCCCAAGAGCAACAACTCAT (CT)19 120–140 PET KJ475540
R: GGCAGTGTTCTATAGGAAATAGTCG
PbL18 F: ATTCATCAACAAGTCTGCCAA (CA)19 84–135 NED KJ475541
R: TGCATTTCTTACTTTTATTTGTGC
PbL21 F: TGTTGCTTTTGCTTTTGCTG (CA)21 269–305 VIC KJ475542
R: CCCTCTGCAGAATTGAGTCC
PbL22 F: TTGCGAATAGGATATGGGTTG (AC)22 164–218 6-FAM KJ475543
R: GAAGACGACAGGCTGCTACA
a

All primers were run at an annealing temperature of 55°C.

b

Based on 40 samples from Pinyiri (syn. Kacheli), Pô (11°14′34.89″N, 1°8′1.73″W), and 40 samples from Tiba, Zitenga (12°42′26.26″N, 1°18′2.04″W), Burkina Faso.

c

Fluorescent tags used to label the 5′ ends of the forward primers.