Table 1.
Locus | Primer sequences (5′–3′) | Repeat motif | Allele size range (bp)b | Fluorescent labelc | GenBank accession no. |
PbL02 | F: CGAAATAAGAACTCGGACCAAA | (GA)17 | 180–205 | NED | KJ475533 |
R: ATGCCGTGTTCTGTTTCACC | |||||
PbL03 | F: TTCGATTCAATTCAACTTGCAG | (GA)17 | 90–138 | 6-FAM | KJ475534 |
R: TCGGTGCTAGCAATATCAGC | |||||
PbL04 | F: GAAGCCTTGGAATGAAGTTGA | (CA)17 | 161–181 | VIC | KJ475535 |
R: GAAAACGGAAGGCATGGTTA | |||||
PbL05 | F: GAATCAGAGAAGCCCTTAGGTT | (AC)17 | 183–259 | PET | KJ475536 |
R: GCCGCTTGTTTTCTTGTGA | |||||
PbL09 | F: TGACGTATTTGAGTGTCTTTTACACA | (AG)18 | 126–170 | 6-FAM | KJ475537 |
R: GCAGAAAATCACAAATGCAGA | |||||
PbL11 | F: TCACGGCAATAGAACTCATCA | (CCT)18 | 170–206 | PET | KJ475538 |
R: ACGGAGCAGGATGAAGTCAG | |||||
PbL12 | F: ATCTAGGCATCCATGCAAATG | (TG)19 | 108–137 | VIC | KJ475539 |
R: CAGAAGTTGTTAGACAATTATGGTGA | |||||
PbL15 | F: CCCCAAGAGCAACAACTCAT | (CT)19 | 120–140 | PET | KJ475540 |
R: GGCAGTGTTCTATAGGAAATAGTCG | |||||
PbL18 | F: ATTCATCAACAAGTCTGCCAA | (CA)19 | 84–135 | NED | KJ475541 |
R: TGCATTTCTTACTTTTATTTGTGC | |||||
PbL21 | F: TGTTGCTTTTGCTTTTGCTG | (CA)21 | 269–305 | VIC | KJ475542 |
R: CCCTCTGCAGAATTGAGTCC | |||||
PbL22 | F: TTGCGAATAGGATATGGGTTG | (AC)22 | 164–218 | 6-FAM | KJ475543 |
R: GAAGACGACAGGCTGCTACA |
All primers were run at an annealing temperature of 55°C.
Based on 40 samples from Pinyiri (syn. Kacheli), Pô (11°14′34.89″N, 1°8′1.73″W), and 40 samples from Tiba, Zitenga (12°42′26.26″N, 1°18′2.04″W), Burkina Faso.
Fluorescent tags used to label the 5′ ends of the forward primers.