Fig. 1.

Raw and processed photographs of Arabidopsis. Unprocessed images (A, D), images after greenest and reddest pixel selection (B, E), and images after final automated processing (C, F) with the delete background option selected. Areas recolored green were identified as leaves and areas recolored red were identified as calibration area. Darker nongreen components in the final image (F) fit pixel threshold and color ratio criteria, but were below the minimum component size, and so were not included in leaf area calculations.