Figure 1.

(A) QrtPCR analysis of endogenous Tac1 gene expression in primary rat amygdala cells treated for 16 h with Dex (n = 3; *p < 0.05). (B) Relative luciferase gene expression driven by either the minimal promoter construct pGL4.23 or TAC1promG-luc plasmids when transfected into primary amygdala neurones (n = 9). (C) Diagrammatic representation (not to scale) demonstrating the linear relationships of the components of each of the different constructs used in the current study. Bent black arrows indicate the transcriptional start site of the luciferase marker gene (Luc). (D) Relative luciferase activity of the constructs represented in C following transfection into amygdala neurones in the presence of vehicle or Dex. In each case n > 3. n.s., not significant; *p < 0.05; **p < 0.01.