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. 2014 Jul 7;6(1):e2014054. doi: 10.4084/MJHID.2014.054

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This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Flow cytometric analysis of erythroid subpopulations at the bone marrow level. (A, B) Primary bone marrow cells were simultaneously stained with CD71+CD45−. We distinguished erythroblast subpopulations (Ery A, Ery B, and Ery C) by considering, in addition to CD71, the forward scatter (FSC) parameter in the gated erythroid population. Ery A is CD71^high FSC^high, Ery B is CD71^high FSC^low, and Ery C is CD71^low FSC^low. (A) Erythroid subpopulation in SCA patients at baseline. (B) Erythroid subpopulation in SCA patients 60 days post-transplant. (C–E) Comparison of percentages of Ery A, Ery B, and Ery C in SCA patients at baseline (gray bar), in SCA patients 60 days post-transplant (black bar) vs. normal donors (white bar) and AS trait carrier donors (dashed bar). The results are expressed as mean ± SD; * p= 0.0028.