Table 2.

Phylogenetic identification of the lipolytic bacteria isolated from original soil (NS) and oil-enriched soil (OS)

Phylogeny assigned	No. of strains isolated	16S rRNA gene sequence comparison
		Species as closest relatives	Accession no.	Identity (%)
NS (non-enriched soil)
Phylum Firmicutes
Bacillus	4	Bacillus simplex DSM 1321	NR_042136	99.6–100
	2	Bacillus megaterium NBRC 13498	AB680420	99.7–100
	1	Bacillus thuringiensis serovar kurstaki HD73	CP004069	99.9
Lysinibacillus	4	Lysinibacillus sphaericus 205y	AF435435	99.7–100
Phylum Proteobacteria
Class Betaproteobacteria
Burkholderia	1	Burkholderia phytofirmans PsJN	CP001052	99.1
Class Gammaproteobacteria
Pseudomonas	1	Pseudomonas arsenicoxydans MaBP1	JQ317810	99.3
	1	Pseudomonas putida ATCC 17472	AF094738	99.6
	1	Pseudomonas putida NBRC 102093	AB681704	98.7
	1	Pseudomonas vancouverensis DhA-51	AJ011507	99.7
Phylum Actinobacteria
Rhodococcus	1	Rhodococcus erythropolis CR-53	AJ786263	99.4
OS (Oil-enriched soil)
Phylum Firmicutes
Bacillus	2	Bacillus thuringiensis serovar kurstaki HD73	CP004069	99.5–99.8
	1	Bacillus simplex DSM 1321	NR_042136	100
Phylum Proteobacteria
Class Betaproteobacteria
Burkholderia	10	Burkholderia caledonica LMG 19076	HQ849076	97.9–99.8
	1	Burkholderia hospita LMG 20598	HQ849087	99.1
	1	Burkholderia glathei N15	NR_037065	97.9
Mitsuaria	1	Mitsuaria chitosanitabida NBRC 102408	AB681764	98.8
Phylum Actinobacteria
Curtobacterium	1	Curtobacterium herbarum DSM 14013	AM410692	96.0