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. 2014 May 23;29(2):154–161. doi: 10.1264/jsme2.ME14002

Table 3.

Phylogenetic identification of the lipolytic metagenomic clones from original soil (NS) and oil-enriched soil (OS)

Phylogeny assigned Clone no. Closest relatives Accession no. Product Identity (%) Family/Groupa
NS (non-enriched soil)
 Alphaproteobacteria NSm01 Parvibaculum lavamentivorans DS-1 YP_001411752 α/β hydrolase 54.3 IV
NSm02 Methylobacterium nodulans ORS 2060 YP_002501216 β-lactamase 47.2 VIII
 Planctomycetes NSm03 Planctomyces maris DSM 8797 ZP_01853484 α/β hydrolase 52.4 IV
NSm04 Pirellula staleyi DSM 6068 YP_003372868 α/β hydrolase 39.9 V
 Acidobacteria NSm05 “Candidatus Solibacter usitatus” Ellin6076 YP_825272 α/β hydrolase 77.8 V
 Unidentified groups NSm06 uncultured bacterium clone pUlp286 ABQ11270 Lipase/esterase 53.8 V
NSm07 uncultured bacterium clone pUlp286 ABQ11270 Lipase/esterase 50.5 V
OS (oil-enriched soil)
 Alphaproteobacteria OSm01 Tistrella mobilis KA081020-065 YP_006374406 β-lactamase 55.0 VIII
OSm02 Caulobacter sp. K31 YP_001682441 β-lactamase 92.2 VIII
OSm03 Methylobacterium radiotolerans JCM 2831 YP_001752969 α/β hydrolase 37.1 IV
OSm04 Methylobacterium nodulans ORS 2060 YP_002498598 β-lactamase 58.7 FE
OSm05 Parvibaculum lavamentivorans DS-1 YP_001411752 α/β hydrolase 58.5 IV
OSm06 Hyphomonas neptunium ATCC 15444 YP_759849 Putative esterase 69.8 VIII
OSm07 Bradyrhizobium diazoefficiens USDA 110 NP_770666 β-lactamase 86.2 VIII
OSm08 Hyphomonas neptunium ATCC 15444 YP_759753 Putative esterase 58.6 VIII
OSm09 Sphingomonas echinoides ATCC 14820 ZP_10338216 β-lactamase 70.8 VIII
OSm10 Mesorhizobium opportunistum WSM2075 ZP_05809008 α/β hydrolase 53.5 V
OSm11 Rhizobium tropici CIAT 899 YP_007335702 α/β hydrolase 50.0 IV
OSm15 Rhodopseudomonas palustris HaA2 YP_486641 Esterase/lipase/thioesterase 59.0 IV
 Betaproteobacteria OSm12 Burkholderia gladioli ATCC 10248 AAF59826 Esterase estB 57.7 VIII
OSm13 Burkholderia gladioli ATCC 10248 AAF59826 Esterase estB 58.1 VIII
OSm14 Burkholderia thailandensis MSMB43 ZP_02464527 Putative lipoprotein 35.4 FE
OSm16 Cupriavidus basilensis OR16 ZP_09622859 β-lactamase 81.4 VIII
 Gammaproteobacteria OSm17 Pseudomonas synxantha BG33R ZP_10142349 Esterase estA 100 II
OSm18 Pseudomonas synxantha BG33R ZP_10142349 Esterase estA 97.3 II
OSm19 Xanthomonas albilineans GPE PC73 YP_003374881 Carboxylesterase bioH 57.7 V
 Deltaproteobacteria OSm20 Desulfococcus oleovorans Hxd3 YP_001530546 β-lactamase 51.1 VIII
 Actinobacteria OSm21 Streptomyces venezuelae ATCC 10712 YP_006876070 Esterase 55.3 VIII
 Armatimonadetes OSm22 Chthonomonas calidirosea T49 YP_008088291 Hydrolase 26.5 V
 Acidobacteria OSm23 “Candidatus Koribacter versatilis” Ellin345 YP_589716 β-lactamase 43.8 VIII
OSm24 “Candidatus Koribacter versatilis” Ellin345 YP_589716 β-lactamase 51.0 VIII
OSm25 “Candidatus Solibacter usitatus” Ellin6076 YP_825272 α/β hydrolase 80.1 V
 Unidentified groups OSm26 uncultured bacterium ACD_17C00118G0001 EKE08525 Hypothetical lipase 50.0 NC
OSm27 uncultured bacterium pELP141 AAS77238 Lipase/esterase 77.2 IV
OSm28 uncultured organism EstC23 AFC77925 Lipase/esterase 74.8 IV
OSm29 uncultured bacterium pELP141 AAS77238 Lipase/esterase 64.3 IV
OSm30 uncultured bacterium pELP141 AAS77238 Lipase/esterase 77.8 IV
OSm31 uncultured bacterium pELP11B AAS77236 Lipase/esterase 76.7 IV
OSm32 uncultured bacterium pELP141 AAS77238 Lipase/esterase 79.5 IV
OSm33 uncultured organism EstC23 AFC77925 Lipase/esterase 77.1 IV
OSm34 uncultured organism EstC23 AFC77925 Lipase/esterase 75.9 IV
OSm35 uncultured bacterium FLS10 ACL67845 Lipolytic enzyme 67.4 IV
OSm36 uncultured bacterium FeKT1 ADH59410 Feruloyl esterase 45.1 FE
OSm37 uncultured bacterium pLE38 AEM45146 Lipase/esterase 84.8 IV
OSm38 uncultured bacterium UVCL29 ACF04196 Lipase/esterase 62.7 IV
OSm39 uncultured bacterium Est24 AFB82697 Lipase/esterase 61.7 IV
a

The definition of the families (or groups) of lipolytic enzymes were according to previous studies (2, 8, 23, 35, 39): II, IV, V, and VIII, number of the family; FE, feruloyl esterase group; NC, novel cluster (shown in Fig. 1 and Fig. S1).