Table 1.
Comparison of sequences between previouly constructed primers and sequences in database at 2007
| Seaquence (5′ to 3′) | No. of sequences | Coverage | ||
|---|---|---|---|---|
|
| ||||
| Archaea | Bacteria | |||
| 530F | GTGCCAGCMGCCGCGG | |||
| 1 | GTGCCAGCAGCCGCGG | 113 | 101,157 | Most of the Bacteria, Methanococcales, some uncultured Archaea |
| 2 | GTGCCAGCCGCCGCGG | 3,518 | 32 | diverse uncultured Archaea* |
| 3 | GTGTCAGCCGCCGCGG | 2,087 | 0 | Crenarchaeota |
| 4 | GTGGCAGCCGCCGCGG | 291 | 0 | Archaeoglobales, Thermococcales, some Korarchaeota |
| 5 | CTGCCAGCCGCCGCGG | 20 | 0 | Methanopyrus, uncultured Euryarchaeota |
| 6 | TTGCCAGCCGCCGCGG | 49 | 0 | some uncultured Halobacteriales & diverse uncultured Euryarchaeota |
| 7 | GTGCCAGCAGCTGCGG | 0 | 390 | Chlamydiaceae & OP5 & diverce bacterial groups |
| 8 | GTGCCAGAAGCGTCGG | 0 | 59 | OP11 |
| 9 | GTGCCAGCAGTCGCGG | 0 | 244 | OP11 & OD1 & diverce bacterial groups |
| 10 | GTGCCAGAAGCCTCGG | 0 | 32 | OP11 & WS6 & OD1 |
| 11 | GTGCCAGAAGAATCGG | 0 | 12 | WS6 |
| 12 | GTGCCAGAAGCATCGG | 0 | 33 | WS6 & OD1 |
| 13 | GTGCCAGCAGCAGCGG | 0 | 132 | WS6 & OP11 & OD1 |
| 14 | GTGCCAGCAGGAGCGG | 0 | 15 | OD1 |
| 15 | GTGGCAGTCGCCACGG | 2 | 0 | Nanoarchaeota |
| 6,080 | 102,106 | |||
| 907R | CCGTCAATTCMTTTRAGTTT | |||
| 1 | CCGTCAATTCCTTTGAGTTT | 0 | 80,234 | Most of Bacteria |
| 2 | CCGTCAATTCCTTTAAGTTT | 37 | 889 | some Korarchaeota & diverse archaeal group |
| 3 | CCGTCAATTCATTTGAGTTT | 1 | 14,881 | diverse bacterial groups |
| 4 | CCGCCAATTCCTTTAAGTTT | 5,877 | 8 | most of Archaea* |
| 5 | CCGCCAATTTCTTTAAGTTT | 73 | 0 | SAGMEG & diverse archaeal group |
| 6 | CCGCCAATTCCTTTGAGTTT | 9 | 740 | some Desulfurococcalesspecies, Aquificae, Thermomicrobia & OP11 |
| 7 | CCGTCAATTCCCTTGAGTTT | 0 | 107 | OP1 & diverse bacterial groups |
| 8 | CCGTCAATTCCTTTATGTTT | 0 | 169 | TM7 & diverse bacterial groups |
| 9 | CCGCCAATTCCTTAAAGTTT | 40 | 0 | uncultured Halobacteriales & several uncultured Archaea |
| 10 | CCGCCAATTCCCTTAAGTTT | 43 | 0 | DSEG & several uncultured archaeal group |
| 11 | CCGTCTATTCCTTTGAGTTT | 0 | 1,809 | Desulfurobacteriaceae & OD1 |
| 12 | CCGTCAATTCATTTAAGTTT | 3 | 24 | Ancient Archaeal Group, uncultured Gammaproteobacteria |
| 13 | CCGTCAATTCCTTCAAGTTT | 31 | 2 | almost Korarchaeota |
| 14 | CCGCCTATTCCTTTAAGTTT | 16 | 0 | Nanoarchaeota |
| 15 | CCGCCAATTCCTTCAAGTTT | 7 | 0 | diverse uncultured archaeal group |
| 16 | CCGCCAATTCCTTTGAATTT | 0 | 11 | OP11 |
| 17 | CCGTCAATTCCTTTGAGCTT | 0 | 83 | diverse bacterial groups |
| 18 | CCGCCAATTCCTTTAAGTAT | 6 | 0 | diverse archaeal groups |
| 19 | CCGCCTATTCCTTTGAGTTT | 0 | 21 | OD1 & some bacterial groups |
| 20 | CCGCCTATCCCTTTGAGTTT | 0 | 11 | OP11 |
| 21 | CCGCCAATTCGTTTGAGTTT | 0 | 26 | OP11 |
| 22 | CCGCCAATTCATTTGAGTTT | 0 | 27 | WS6 |
| 23 | CCGTCAATTTCTTTGAGTTT | 0 | 2,580 | Some of Deltaproteobacteria & diverse bacterial groups |
| 24 | CCGTCAATTCTTTTGAGTTT | 0 | 572 | Planctomycetes, Chlomadiae |
| 25 | CCGTCAATTCGTTTGAGTTT | 0 | 142 | EM19, uncultured Chloroflexi |
| 26 | CCGCCAATTCCCTTAAGTTT | 43 | 0 | SAGMEG |
| 6,186 | 102,336 | |||
*
All of the ANME related seqences are included in these group without any mismatch residues.