Table 2.
Prokaryotic SSU rRNA gene primer sequences constructed in this study
| Name | Sequence (5′-3′) | Group coverage | Proportion in mixtures |
|---|---|---|---|
| Prokaryotic set | |||
| 30F mixture | |||
| Bac 530F | GTGCCAGCAGCCGCGG | 1 | 30 |
| Arch 530F | GTGBCAGCCGCCGCGG | 2, 3, 4 | 24 |
| Arch2 530F | YTGCCAGCCGCCGCGG | 5, 6 | 6 |
| Bac2 530F | GTGCCAGCAGCWGCGG | 7, 13, 14 | 1 |
| Bac3 530F | GTGCCAGCAGTCGCGG | 9 | 1 |
| Bac4 530F | GTGCCAGAAGMMTCGG | 10, 11, 12, (8) | 1 |
| Nano 530F | GTGGCAGTCGCCACGG | 15 | 3 |
| 907R mixture | |||
| Uni 907R | CCGYCAATTCMTTTRAGTTT | 1, 2, 3, 4, 6, 12, 19, 22, (8, 17, 18, 23, 24, 25) | 20 |
| DeepAB 907R | CCGYCTATTCCTTTGAGTTT | 11, (14), 20 | 1 |
| SAG-Del 907R | CCGYCAATTTCTTTRAGTTT | 5, 23 | 1 |
| DeepAB2 907R | CCGYCAATTCCCTTRAGTTT | 7, 10, 26 | 1 |
| Arch2 907R | CCGYCAATTCCTTMAAGTTT | 9, 13, 15 | 1 |
| OP11 907R | CCGCCAATTCCTTTGAATTT | 16, 21 | 1 |
Numbers in group coverage are refered from Table 1.
Groups in parentheses harbor 1 base mismatch with primer sequence.