Table 3.

Coverage of 530F and 907R primers targeting SILVA SSURef database at the end of 2011. In the database, we identified archaeal 13,022, badterial 282,285 and eukariotic 36,054 sequences that harbor both 530F and 907R regions

(A) Number of sequences without mismatch residues compared to 530F primers.
530F primers	Archaea	Bacteria	Eukarya
Arch_530F	12,062	63	78
Arch2_530F	90	3	0
Nano_530F	38	0	0
Bac_530F	214	271,887	33,165
Bac2_530F	0	1,031	502
Bac3_530F	0	562	38
Bac4_530F	0	188	1
Total	12,404	273,734	33,784
Coverage (%) without mismatch residues	95.3	97.0	93.7
(B) Number of sequences with 1 mismatch residues compared to 530F primers.
530F primers	Archaea	Bacteria	Eukarya
Arch_530F	298	490	49
Arch2_530F	12	222	16
Nano_530F	9	0	0
Bac_530F	19	3,979	1,056
Bac2_530F	7	174	39
Bac3_530F	2	151	39
Bac4_530F	0	169	14
Total	347	5,185	1,213
Coverage (%) with 1 mismatch residue	2.7	1.8	3.4
(C) Number of sequences without mismatch residues compared to 907R primers.
907R primers	Archaea	Bacteria	Eukarya
Uni_907R	11,166	256,453	27,714
DeepAB_907R	0	3,835	0
SAG-Del_907R	158	6,020	5,679
DeepAB2_907R	138	428	25
Arch2_907R	159	4	368
OP11_907R	0	7	0
Total sequences	11,621	266,747	33,786
Coverage (%) without mismatch residues	89.2	94.5	93.7
(D) Number of sequences with 1 mismatch residues compared to 907R primers.
907R primers	Archaea	Bacteria	Eukarya
Uni_907R	363	7,790	622
DeepAB_907R	1	760	31
SAG-Del_907R	17	437	420
DeepAB2_907R	27	225	7
Arch2_907R	13	256	72
OP11_907R	6	279	0
Total sequences	427	9,747	1,152
Coverage (%) with 1 mismatch residue	3.3	3.5	3.2