Fig. 1.

Phylogenetic tree based on 16S rRNA gene sequences for nitrogen-fixing enterobacteria associated with sugarcane cultivar ROC22 and the type strains of the species belonging to the genera Enterobacter and Klebsiella. Accession numbers of the 16S rRNA genes in the GenBank database are given in parentheses. The tree was generated by the maximum-likelihood principle using the PhyML 3.0 program. The TIM1+G model was used. Branch support measure was assessed by a Shimodaira-Hasegawa-like test and values >50 are indicated at the nodes. Bar, 0.01 nucleotide substitutions per site.