Table 2.
Tetracycline resistance and presence of resistance genes in the environmental isolates
| Isolate | BOX group | Identificationa | TET resistance (zone in mm)b | Presence of genec | |
|---|---|---|---|---|---|
|
| |||||
| tap | tet(V) | ||||
| Site3-B14 | A | M. litorale (99.46%) | 12 | + | + |
| Site3-B33 | A | M. septicum (100%) | 35 | + | + |
| Site4-B4 | C | M. alvei (98.99%) | 9 | + | + |
| Site4-B30 | C | M. septicum (100%) | 12 | + | + |
| Site4-B31 | D | M. septicum (99.06%) | 11 | + | + |
| Site1-IIA/46 | E | M. septicum (99.84%) | 12 | + | + |
| Site4-B5 | F | M. septicum (99.58%) | 20 | + | + |
| Site4-B18 | F | ND | 25 | + | + |
| Site4-B19 | F | ND | 18 | + | + |
| Site1-8A | F | M. septicum (99.93%) | 14 | + | + |
| Site1-10A | F | M. septicum (100%) | 13 | + | + |
| Site4-B2 | G | M. septicum (100%) | 11 | + | − |
| Site4-B7 | G | ND | 15 | + | − |
| Site4-B8 | G | ND | 9 | − | − |
| Site4-B29 | G | M. septicum (99.77%) | 11 | + | + |
| Site2-2C | H | M. septicum (100%) | 14 | + | + |
| Site2-3C | H | M. septicum (100%) | 14 | + | + |
| Site2-5C | H | M. septicum (99.78%) | 17 | + | + |
| Site2-7C | H | M. septicum (99.76%) | 10 | + | + |
| Site4-B1 | I | M. septicum (99.55%) | 11 | + | + |
| Site4-B3 | I | M. septicum (98.93%) | 37 | + | + |
| Site4-B9 | I | M. septicum (99.25%) | 11 | + | + |
| Site4-B23 | I | ND | 10 | + | + |
| Site4-B24 | I | ND | 39 | + | + |
| Site4-B26 | I | M. fortuitum subsp. acetamidolyticum (99.46%) | 35 | + | + |
| Site4-B27 | I | ND | 35 | + | + |
| Site4-B28 | I | ND | 10 | + | + |
| Site2-4C | J | M. fortuitum subsp. acetamidolyticum (99.42%) | 31 | + | + |
| Site4-B6 | M | M. septicum (99.17%) | 10 | − | − |
| Site4-B16 | M | ND | 9 | − | − |
| Site4-B17 | M | ND | 8 | − | − |
| Site4-B21 | M | ND | 9 | − | − |
| Site4-B39 | M | ND | 20 | − | − |
| Site4-B15 | P | M. septicum (99.71%) | 50 | + | + |
| Site4-B25 | P | M. septicum (99.56%) | 55 | + | + |
| Site4-B38 | Q | M. septicum (100%) | 14 | + | + |
| Site1-2A | Q | M. septicum (99.71%) | 53 | + | + |
| Site1-3A | Q | M. septicum (99.71%) | 56 | + | + |
| Site1-9A | Q | M. septicum (99.71%) | 56 | + | + |
| Site1-11A | Q | M. septicum (99.71%) | 52 | + | + |
| Site3-B10 | U | M. septicum (100%) | 15 | + | − |
| Site3-B34 | U | ND | 10 | + | − |
| Site4-B36 | U | M. septicum (99.67%) | 10 | + | − |
| Site2-IIIC/14 | δ | M. aubagnense (99.04%) | 12 | − | − |
a
In parentheses, % pairwise sequence similarity with the closest type strain on EzTaxon is shown. ND, not done.
b
Resistance in bold.
c
The genes otr(A), otr(B), tet(K)(L) and tet(M) were detected in none of the isolates.