Fig. 7.

Restored growth speed and motility of R18DOX aposymbiotic amoebae by re-infection with eR18 Protochlamydia.

(A)Representative DAPI staining images of wild-type R18WT amoebae (WT) R18 aposymbiotic amoebae before (DOX) and after re-infection (Re-infection). Amoebae were observed using a conventional fluorescence microscope at 1,000× magnification. Arrows show representative bacterial clusters. N, amoebal nucleus. (B and C) Effect on growth speed (B) and motility (C) of R18DOX aposymbiotic amoebae of re-infection with Protochlamydia. R18DOX aposymbiotic amoebae were re-infected with eR18 Protochlamydia at a multiplicity of infection of 10. The data show the mean ± SD of at least three independent experiments. *P<0.05 vs. cultures with R18WT amoebae (Student’s t-test with Bonferroni correction).