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. Author manuscript; available in PMC: 2015 May 27.
Published in final edited form as: Dev Cell. 2014 May 15;29(4):454–467. doi: 10.1016/j.devcel.2014.04.011

Figure 6. Changes in FGF signaling cannot induce cell fate switch after PrE and EPI specification.

Figure 6

A Experimental timeline. B Representative immunofluorescence images of Gata6+/+ and Gata6−/− embryos cultured from the salt-and-pepper (E3.5) stage for 24h in medium without (untreated) or with 500ng/ml FGF4 + 1μg/ml Heparin (+FGF4) or medium + 1μM PD0325901 (ERKi). Blue: Hoechst; white: NANOG; red: GATA6. C Percentages of embryos falling into 1 of five FGF-response categories: 0% EPI, 1–20% EPI, 21–40% EPI, 41–60% EPI and >60% EPI in ICMs of embryos treated as in (A). EPI: Epiblast.