Figure 4.

NRL knockdown decreased Reep6 promoter activity in adult mouse retina. Representative confocal images of P21 CD1 mouse retinas, electroporated in vivo at P0 with the Reep6-driven GFP reporter construct (CIR-P752-GFP). mCherry construct driven by CAG promoter (CAG-mCherry) was used as electroporation control. Retina co-transfected with Gapdh shRNA control and GFP reporter showed green signal (top), whereas Nrl-shRNA transfection greatly eliminated Reep6 driven green signal (bottom). GFP, mCherry and DAPI are shown in green, red and blue, respectively. ONL, outer nuclear layer. Scale bar = 10 μm.