Figure 6.

Expression analyses in heparanase 2 knock-down embryos. RNA from pools of Stages 32 and 40 control (CTL MO) and morphant (hpse2 MO1) embryos was subjected to RT-PCR, with serial dilutions of cDNA depicted on the right of each row. drosha, which encodes an RNase III enzyme, was used as a housekeeping control. Morphants had: downregulated wt hpse2 exon 2 and the appearance of the shorter exon 2Δ amplicon; increased levels of hpse1 and lrig2; increased levels of fgf2 and of the neuronal precursor markers olig2 (at Stage 40) and nkx6.1; upregulated myod1, encoding a skeletal muscle transcription factor, and downregulated myh11, encoding a smooth muscle myosin (at Stage 40). Levels of syn1 and chrnb2, encoding synaptic molecules, were similar in morphants and controls.