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. 2014 Apr 1;23(16):4302–4314. doi: 10.1093/hmg/ddu147

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© The Author 2014. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — pERK detected by western blotting and IHC. (A) Western blots for pERK and tERK in sets of pooled embryos collected, as indicated, between Stages 30 and 41. Note that the signal for pERK was clearly increased in heparanase 2 knock-down embryos versus CTL MO embryos between Stages 30 and 36. This effect was no longer apparent at Stages 40 and 41 (B–E) Transverse section of the neural tube of Stage 32 uninjected embryo, with the dorsal surface uppermost, showing (B) all nuclei stained with diamindino-2-phenylindole (DAPI), (C) heparanase 2 immunoreactivity, (D) pERK immunoreactivity, and (E) merged image. pERK+ nuclei (two are indicated by arrows) and heparanase 2+ cells (two are indicated by arrowheads) are detected in the lateral zones of the tube. Note that the cells with strong nuclear pERK immunostaining and those with strong heparanase 2 signals tend to be mutually exclusive. The image is representative of experiments on three embryos.