Table 1.
Genotype | Transgene | N | Muv (%) | Vul (%) | Pvl (%) | N | P6.p induction (%) |
---|---|---|---|---|---|---|---|
wild-type | none | 207 | 0 | 0 | 1.0 | 48 | 100 |
let-60(n1046) | none | 201 | 77.9 | – | 0.5 | 50 | 100 |
let-60(n1046) | ras-1wt | 244 | 76.4 | – | 2.8 | 43 | 100 |
let-60(n1046) | ras-1G27dup | 231 | 87.1a | – | 3.0 | 50 | 100 |
let-23(sy1) | none | 194 | – | 87.8 | 3.6 | 178 | 13.4 |
let-23(sy1) | ras-1WT | 169 | – | 84.3 | 4.1 | 156 | 14.0 |
let-23(sy1) | ras-1G27dup | 282 | – | 83.3 | 10.3b | 128 | 24.2c |
Strains: let-60(n1046) is a gain-of-function allele of let-60 (ortholog of the human HRAS, KRAS and NRAS genes); let-23(sy1) is a hypomorphic allele of let-23 (ortholog of the human EGFR gene). ras-1WT and ras-1G27dup indicate hsp-16.41::ras-1WT- and hsp-16.41::ras-1G27dup-containing constructs injected at 100 ng/μl, respectively. After each cross, isogenic worms that had lost the transgene were cloned separately and used as controls.
Animals were grown at 20°C and heat-shocked in parallel at early L3 stage. N indicates the number of animals scored. Multivulva (Muv), vulvaless (Vul) and protruding vulva (Pvl) phenotypes are expressed as percentage of worms with ectopic pseudovulvae, animals lacking a vulva and adults with a protruding vulva, respectively. Induction of vulval cell fate is expressed as percentage of P6.p that has been induced to invaginate.
In all comparisons, P-values were calculated using two-tailed Fisher's exact test.
aSignificantly different from let-60(n1046) (P < 0.02).
bSignificantly different from let-23(sy1) (P < 0.01) and let-23(sy1);ras-1WT (P < 0.02).
cSignificantly different from let-23(sy1) (P = 0.02) and let-23(sy1);ras-1WT (P < 0.05).