Figure 3. Abundance of photorespiratory transcripts and proteins in leaves of individual Flaveria species.
Normalized transcript (A) and protein (B) amounts are plotted as heat maps. Transcript amounts were determined by Illumina sequencing of the leaf transcriptomes and read mapping on selected F. robusta full length transcript sequences. Protein amounts were determined by protein gel blots. See Figure 3—source data 1 for absolute transcript levels, Figure 3—source data 2 for protein quantification and Figure 3—figure supplements 1 and 2 for immunoblots. Fp: F. pringlei (C3); Fro: F. robusta (C3); Fc: F. chloraefolia (C3–C4); Fpu: F. pubescens (C3–C4); Fa: F. anomala (C3–C4); Fra: F. ramosissima (C3–C4); Fbr: F. brownii (C4-like); Fb: F. bidentis (C4); Ft: F. trinervia (C4).
DOI: http://dx.doi.org/10.7554/eLife.02478.007
Figure 3—source data 1. Transcript abundance of photorespiratory genes determined by read mapping on F. robusta full length transcript sequences.
elife02478s003.xlsx (81KB, xlsx)
DOI: 10.7554/eLife.02478.008
Figure 3—source data 2. Quantification of photorespiratory proteins by protein gel blot.
elife02478s004.xlsx (36.9KB, xlsx)
DOI: 10.7554/eLife.02478.009
Figure 3—figure supplement 1. Results of the protein analyses.
30 µg of total protein was electrophoresed on a polyacrylamide-SDS gel and stained with coomassie blue as control of total protein concentrations. Fp: F. pringlei (C3); Fro: F. robusta (C3); Fc: F. chloraefolia (C3–C4); Fpu: F. pubescens (C3–C4); Fa: F. anomala (C3–C4); Fra: F. ramosissima (C3–C4); Fbr: F. brownii (C4-like); Fb: F. bidentis (C4); Ft: F. trinervia (C4).
Figure 3—figure supplement 2. Results of the protein analyses.
Immunoblot results with antibodies against photorespiratory proteins (C) Immunoblot results with antibodies against C4 proteins. Fp: F. pringlei (C3); Fro: F. robusta (C3); Fc: F. chloraefolia (C3–C4); Fpu: F. pubescens (C3–C4); Fa: F. anomala (C3–C4); Fra: F. ramosissima (C3–C4); Fbr: F. brownii (C4-like); Fb: F. bidentis (C4); Ft: F. trinervia (C4).