Table 2.
Target gene | Primer sequence (5′→3′) |
---|---|
β-Actin | F: CAC CAG TTC GCC ATG GAT |
R: ATC ACA CCC TGG TGC CTA | |
Cu/Zn-SOD | F: GTC GTC TCC TTG CTT TTT GC |
R: TCT GCT CGA AGT GAA TGA CG | |
Mn-SOD | F: CGT CAC CGA GGA GAA GTA CCA CGA |
R: CAG CCT GAA CCT TGG ACT CCC ACA | |
GPx | F: GAA GGT AAA GAG CGG GTG AG |
R: AGG AGA ATG GCA AGA ATG AAG | |
CAT | F: AAG CTG GTT AAT GCG AAT GG |
R: CAA GTT TTT GAT GCC CTG GT | |
NF-κB (p65) | F: ACG ATC TGT TTC CCC TCA TC |
R: CAC TTG TAA CGG AAA CGC AT | |
COX-2 | F: ACT ACG CCG AGA TTC CTG AC |
R: CAA TGT TCC AGA CTC CCT TG | |
iNOS | F: GTG TTC CAC CAG GAG ATG TT |
R: ACC GCT TTC ACC AAG ACT G | |
IL-1β | F: GCC TCA AGG GGA AGA ATC TAT |
R: CAA ACC GCT TTT CCA TCT TCT | |
TNF-α | F: GAG CTC AAG CCC TGG TAT G |
R: CGG ACT CCG TGA TGT CTA AG |
SOD, superoxide dismutase; GPx, glutathione peroxidase; CAT, catalase; NF-κB, necrosis factor-kappaB; COX, cyclooxygenase; iNOS, inducible nitric oxide synthase; IL, interleukin; TNF-α, tumor necrosis factor-alpha.