Table 3.
Sulforaphane contents of an 80% methanol extract and organic solvent fractions from broccoli florets and the ability of the extract and fractions to inhibit nitric oxide production by LPS (100 ng/mL)-induced RAW 264.7 cells
| Extract and fractions | Sulforaphane content (μg/g) | TC501) (μg/mL) | IC502) (μg/mL) | Selectivity index3) |
|---|---|---|---|---|
| 80% Methanol extract | 60.6±5.1 | >1,000 | 777.3±17.9 | 1.28 |
| n-Hexane fraction | ND4) | 81.3±10.0 | 24.3±1.1 | 3.34 |
| Ethyl acetate fraction | 620.2±14.1 | 192.3±6.7 | 46.6±0.5 | 4.12 |
| n-Butanol fraction | 272.7±21.7 | >1,000 | >1,000 | 1 |
| Distilled water fraction | ND | >1,000 | >1,000 | 1 |
1)
TC50, the concentration of extract or fraction needed to reduce cell viability by 50%.
2)
IC50, the concentration of extract or fraction needed to reduce nitric oxide production by 50%.
3)
Selectivity index=TC50/IC50.
4)
ND, not detected.