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. 2014 Jun 19;25(7):1203–1212. doi: 10.1021/bc500240p

Figure 4.

Figure 4

Development of a continuous FRET assay for PFTase: (Above) Emission spectra of a reaction mixture containing GFP-CVIA and coumarin FPP analogue before and after the addition of PFTase showing overall decrease in the fluorescence intensity of the coumarin at 460 nm with simultaneous increase in the fluorescence intensity of GFP at 510 nm (λex = 330 nm). (Below) Continuous FRET assay based on labeling of GFP with the coumarin analogue showing an increase in the fluorescence intensity at 510 nm after the addition of PFTase (λex = 330 nm).