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Labeling of CNTF with NBD analogue 5 by wild-type and Y205Aβ mutant PFTase. Fluorescence intensity of the fluorescence scan shown in the SI was calculated using ImageJ software. Conversion is based on the ratio of fluorescence at the given time point to the fluorescence at its maximum. The lines on the graph indicate the initial rate of enzyme-catalyzed NBD incorporation determined in the first 15 min of reaction.
