. 2014 Jun 19;25(7):1203–1212. doi: 10.1021/bc500240p

Table 2. Kinetic Values for PFTase and Three Mutants Using FPP as the Isoprenoid Substrate^a.

protein	k_cat (s^–1)	K_M (μM)	k_cat/K_M (μM s^–1)	rel. k_cat/K_M
WT	0.27 ± 0.02	0.5 ± 0.2	0.5 ± 0.2	1
W102A	0.083 ± 0.005	0.26 ± 0.09	0.3 ± 0.1	0.59
Y154A	0.11 ± 0.007	0.27 ± 0.09	0.4 ± 0.1	0.76
Y205A	0.18 ± 0.01	0.5 ± 0.2	0.4 ± 0.1	0.67

All values are apparent kinetic parameters since the peptide concentration was held constant at 2 μM. Values are reported here as the averages and standard deviations from three separate experiments.

Table 2. Kinetic Values for PFTase and Three Mutants Using FPP as the Isoprenoid Substratea.

Table 2. Kinetic Values for PFTase and Three Mutants Using FPP as the Isoprenoid Substrate^a.