. 2014 Jun 19;25(7):1203–1212. doi: 10.1021/bc500240p

Table 3. Kinetic Values for PFTase and Three Mutants Using the Benzaldehyde Containing Analogue (2) as the Substrate^a.

protein	k_cat (s^–1)	K_M (μM)	k_cat/K_M (μM s^–1)	rel. k_cat/K_M
WT	0.0021 ± 0.0002	2.4 ± 0.6	0.0008 ± 0.0002	1
W102A	0.0076 ± 0.0005	0.2 ± 0.1	0.04 ± 0.02	43
Y154A	0.0014 ± 0.0009	0.7 ± 0.2	0.002 ± 0.001	2.3
Y205A	0.0012 ± 0.0006	0.5 ± 0.1	0.002 ± 0.001	2.7

All values are presented as apparent kinetic parameters with the peptide concentration held constant at 2 μM. Values are reported here as the averages and standard deviations from three separate experiments.

Table 3. Kinetic Values for PFTase and Three Mutants Using the Benzaldehyde Containing Analogue (2) as the Substratea.

Table 3. Kinetic Values for PFTase and Three Mutants Using the Benzaldehyde Containing Analogue (2) as the Substrate^a.