. 2014 Jun 19;25(7):1203–1212. doi: 10.1021/bc500240p

Table 4. Kinetic Values for PFTase and the Y205A Mutant Using the Cyclo-Octene Containing Analogue (3) as the Substrate^a.

protein	k_cat (s^–1)	K_M (μM)	k_cat/K_M (μM s^–1)	rel. k_cat/K_M
WT	0.017 ± 0.002	0.2 ± 0.1	0.08 ± 0.04	1
Y205A	0.082 ± 0.007	0.5 ± 0.2	0.16 ± 0.07	2

All values are presented as kinetic parameters with the peptide concentration held constant at 2 μM. Values are reported here as the averages and standard deviations from three separate experiments.

Table 4. Kinetic Values for PFTase and the Y205A Mutant Using the Cyclo-Octene Containing Analogue (3) as the Substratea.

Table 4. Kinetic Values for PFTase and the Y205A Mutant Using the Cyclo-Octene Containing Analogue (3) as the Substrate^a.