. 2014 Jun 19;25(7):1203–1212. doi: 10.1021/bc500240p

Table 5. Kinetic Values for PFTase and Three Mutants Using Coumarin Containing Analogue (4) as the Substrate^a.

protein	k_cat (min^–1)	K_m (μM)	k_cat/K_M (μM min^–1)	rel. k_cat/K_M
WT	0.08 ± 0.01	1.4 ± 0.3	0.06 ± 0.01	1
Y205A	2.1 ± 0.3	0.12 ± 0.08	20 ± 10	300

These values were generated using the PFTase FRET assay described herein. All values are reported as apparent kinetic parameters with the GFP-CVIA concentration held constant at 2.5 μM. Values are reported here as the averages and standard deviations from three separate experiments.

Table 5. Kinetic Values for PFTase and Three Mutants Using Coumarin Containing Analogue (4) as the Substratea.

Table 5. Kinetic Values for PFTase and Three Mutants Using Coumarin Containing Analogue (4) as the Substrate^a.