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. 2014 Jul 18;9(7):e101200. doi: 10.1371/journal.pone.0101200

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© 2014 Saldaña et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunogold labeling of ECP produced by (a) CFT073, (b) CFT073 ΔecpA, (c) CFT073 ΔecpA (pMAT9), (d) F11, (e) F11 ΔecpA, and (f) F11 ΔecpA (pMAT9) with anti-ECP antibodies and rabbit anti-IgG gold conjugate. Scale bars represent 500 nm. (B) Immunofluorescence of UPEC strains with anti-ECP antibodies and rabbit anti-IgG and Alexa-Fluor 488-conjugated secondary antibody (green). Cellular and bacterial DNA was stained with propidium iodide (red). Images were taken with a 100× objective and magnified 5X. (C) Immunoblot showing EcpA production in CFT073, CFT073 ΔecpA (pMAT9), F11 and F11 ΔecpA (pMAT9), but not in the CFT073 ΔecpA or F11 ΔecpA mutants. DnaK detection was used as loading control of equal amounts of bacteria onto the gels. (D) Motility of UPEC strains in motility agar. Except for the CFT073 ΔfliC all other strains showed motility.